Plasmid

Part:BBa_K1321309:Experience

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

This experience page is provided so that any user may enter their experience using this part.

pBAV1K-T5-gfp replciation has been confirmed in E.coli and G.xylinus by miniprepping transformed cells (both E.coli and G.xylinus, and using purified plasmid DNA as template for PCR with pBAV1K-sepcific primers. NOTE: sequencing results differ from the sequence provided by the original authors, showing a deletion in the double lac operator, however, sequence uploaded in the registry shows the deletion and matches the sequence of the part.

Applications of BBa_K1321309

pBAV1k-T5-GFP works in Acinetobacter baylyi ADP1 strain (Murin CD, Segal K, Bryksin A, Matsumura I. Expression Vectors for Acinetobacter baylyi ADP1. Applied and Environmental Microbiology. 2012;78(1):280-283. doi:10.1128/AEM.05597-11.). Its experimental characterization can be found below. Codon-optimized GFP (for Acinetobacter baylyi ADP1) shows higher expression than existing GFP. The y-axis values have been normalized relative to BBa K1321309. <img src="GFP_correct.png">

User Reviews

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